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Tuesday, 20 October 2009 08:55 |
Multiple-wavelength anomalous diffraction (MAD) and macromolecular crystallography (MX)
Scientific discipline: Structural biology
| GENERAL BEAMLINE INFORMATION |
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Operational
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Yes
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| Proposal cycle |
Proposals for Structural Biology Beamlines (2-month cycle) |
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Source characteristics
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Superbend magnet (5.0 tesla, single pole)
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Energy range
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5-17 keV (1% max flux)
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Monochromator
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Double flat crystal, Si(111)
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Measured flux (1.9 GeV, 400 mA)
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2.5 x 1011 at 11 keV
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Resolving power (E/ΔE)
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7,000
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| Divergence (max at sample) |
3.0 (h) x 0.35 (v) mrad |
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Endstations
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Minihutch
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Detectors
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3 x 3 CCD array (ADSC Q315r)
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Measured spot size at sample (FWHM)
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0.120 (h) x 0.108 (v) mm
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Sample format
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Single crystals of biological molecules. Crystallization tray goniometer available with prior arrangement.
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Sample preparation
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Support labs available
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Sample environment
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Ambient or ~100 K
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Special notes
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Computers for data processing and analysis, robotic sample handling available
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| Scientific disciplines |
Structural biology |
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Scientific applications
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Biological crystallography; multiple-wavelength anomalous diffraction (MAD), monochromatic protein crystallography
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Local contact
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James Holton
UCSF/Physical Biosciences Division, Berkeley Lab
Phone: (510) 486-4587
Fax: (510) 486-5298
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Physical Biosciences Division, Berkeley Lab
Phone: (510) 495-2249
Fax: (510) 486-5298
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Physical Biosciences Division, Berkeley Lab
Phone: (510) 495-2404
Fax: (510) 486-5298
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Spokesperson
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University of California, Berkeley
Phone: (510) 666-2749
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Beamline phone number
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(510) 495-2083
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| Website |
http://bl831.als.lbl.gov/  |
| ENDSTATION INFORMATION |
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Endstation name
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Macromolecular crystallography
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| Spot size at sample |
10-100 µm (collimated) |
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Detector
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3 x 3 CCD array (ADSC Q315r)
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| Sample format |
Single crystals of biological molecules, all sample pin types and lengths supported |
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Sample preparation
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Support labs available
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Sample environment
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100-300 K
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Scientific applications
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Macromolecular crystallography, multiple-wavelength anomalous diffraction (MAD)
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| Sample handling automation |
"Cool Hand Luke" robot accepts all major pin types (Hampton, Yale, SPINE, etc.) and lengths (10-24 mm) and supports "delayed data collection mode" where data collection runs are deferred to the end of the shift. This robot does not work directly with cassettes because hand-mounting of samples in the mini-hutch is faster than any robot. |
| ENDSTATION INFORMATION |
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Endstation name
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Tray Goniometer
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| Spot size at sample |
50 µm default |
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Detector
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3 x 3 CCD array (ADSC Q315r)
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| Characteristics |
Optional unit may be installed in hutch upon advance request. |
| Sample format |
SBS compliant crystallization trays oriented 90 degrees to gravity. Trays that limit the amount of plastic in the beam to roughly the size of the crystals are highly recommended. |
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Sample preparation
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Advance notice required. It takes 1 hour of day shift to install the tray goniometer and ~30 minutes to return to cryo-PX.
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Sample environment
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Ambient
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Scientific applications
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Rapid screening of crystallization trials for diffraction.
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About the ALS 
