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Beamline 8.3.1 Print
Multiple-wavelength anomalous diffraction (MAD) and macromolecular crystallography (MX)

 

Scientific discipline: Structural biology

 

GENERAL BEAMLINE INFORMATION

Operational

Yes

Proposal cycle Proposals for Structural Biology Beamlines (2-month cycle)

Source characteristics

Superbend magnet (5.0 tesla, single pole)

Energy range

5-17 keV (1% max flux)

Monochromator

Double flat crystal, Si(111)

Measured flux (1.9 GeV, 400 mA)

2.5 x 1011 at 11 keV

Resolving power (E/ΔE)

7,000

Divergence (max at sample) 3.0 (h) x 0.35 (v) mrad

Endstations

Minihutch

Detectors

3 x 3 CCD array (ADSC Q315r)

Measured spot size at sample (FWHM)

0.120 (h) x 0.108 (v) mm

Sample format

Single crystals of biological molecules. Crystallization tray goniometer available with prior arrangement.

Sample preparation

Support labs available

Sample environment

Ambient or ~100 K

Special notes

Computers for data processing and analysis, robotic sample handling available

Scientific disciplines Structural biology

Scientific applications

Biological crystallography; multiple-wavelength anomalous diffraction (MAD), monochromatic protein crystallography

Local contact

James Holton
UCSF/Physical Biosciences Division, Berkeley Lab
Phone: (510) 486-4587
Fax: (510) 486-5298

 

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Physical Biosciences Division, Berkeley Lab
Phone: (510) 495-2249
Fax: (510) 486-5298

 

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Physical Biosciences Division, Berkeley Lab
Phone: (510) 495-2404
Fax: (510) 486-5298

Spokesperson

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University of California, Berkeley
Phone: (510) 666-2749

Beamline phone number

(510) 495-2083

Website http://bl831.als.lbl.gov/

 

ENDSTATION INFORMATION

Endstation name

Macromolecular crystallography

Spot size at sample 10-100 µm (collimated)

Detector

3 x 3 CCD array (ADSC Q315r)

Sample format Single crystals of biological molecules, all sample pin types and lengths supported

Sample preparation

Support labs available

Sample environment

100-300 K

Scientific applications

Macromolecular crystallography, multiple-wavelength anomalous diffraction (MAD)

Sample handling automation "Cool Hand Luke" robot accepts all major pin types (Hampton, Yale, SPINE, etc.) and lengths (10-24 mm) and supports "delayed data collection mode" where data collection runs are deferred to the end of the shift. This robot does not work directly with cassettes because hand-mounting of samples in the mini-hutch is faster than any robot.

 

ENDSTATION INFORMATION

Endstation name

Tray Goniometer

Spot size at sample 50 µm default

Detector

3 x 3 CCD array (ADSC Q315r)

Characteristics Optional unit may be installed in hutch upon advance request.
Sample format SBS compliant crystallization trays oriented 90 degrees to gravity. Trays that limit the amount of plastic in the beam to roughly the size of the crystals are highly recommended.

Sample preparation

Advance notice required. It takes 1 hour of day shift to install the tray goniometer and ~30 minutes to return to cryo-PX.

Sample environment

Ambient

Scientific applications

Rapid screening of crystallization trials for diffraction.