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Beamline 12.3.1 Print
Tuesday, 20 October 2009 09:33

Structurally Integrated Biology for Life Sciences (SIBYLS)

Scientific discipline: Structural biology

GENERAL BEAMLINE INFORMATION

Operational

Yes

Proposal cycle Proposals for Structural Biology Beamlines (2-month cycle)

Source characteristics

Superbend

Energy range

5-17 keV (1% max flux)

Frequency range 2.5-0.73 Angstrom wavelength
Beam size

100 µm round beam default
10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively.
120 µm x 120 µm Gaussian uncollimated beam shape at sample.

Scientific discipline Structural biology

Monochromator #1

Si(111) Double crystal

Calculated flux (1.9 GeV, 400 mA)

2.5 x 1011 photons/sec at 11 keV

Resolving power (E/ΔE)

7000

Monochromator #2

0.6% bandpass multilayers

Calculated flux (1.9 GeV, 400 mA)

4 x 1013 photons/s with a convergence angle of 3 mrad

Resolving power (E/ΔE)

100

Special notes

Computers for data processing and analysis are available.

Local contacts

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Physical Biosciences Division, Berkeley Lab
Phone: (510) 486-5378
Fax: (510) 486-5298

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Physical Biosciences Division, Berkeley Lab
Phone: (510) 495-2697
Fax: (510) 486-5298

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Physical Biosciences Division, Berkeley Lab
Phone: (510) 486-5378
Fax: (510) 486-5298

Spokesperson

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The Scripps Research Institute
Phone: (858) 784-8119
Fax: (858) 784-2289

Beamline phone number (510) 495-2134
Website SIBYLS: http://bl1231.als.lbl.gov/

ENDSTATION INFORMATION

Endstation name

Macromolecular crystallography

Detector

3 x 3 array CCD, ADSC Q315

Spatial resolution ~1 µm pixel video microscope

Calculated spot size at sample (FWHM)

10-100 µm (collimated)

Sample format

Single-crystal biomolecules

Sample preparation

Support labs available (Bldg. 6, Rm. 2105)

Sample environment

100-300K

Scientific applications

Macromolecular crystallography, multiple-wavelength anomalous diffraction (MAD)

ENDSTATION INFORMATION

Endstation name

Small-angle X-ray scattering

Detector

Mar 165 CCD

Calculated spot size at sample (FWHM)

~5.0 x 0.5 mm

Sample format

~15 µL solutions of macromolecules and buffer blanks

Sample preparation

Support labs available (Bldg. 6, Rm. 2105)

Sample environment

80 - 4C, anaerobic

Scientific applications

High-throughput solution structural analyses by SAXS.
Solution structure modeling, molecular envelope shape reconstruction, identification and characterization of oligomeric states and macromolecular complex assembly.

More information about Beamline 12.3.1 is available on the SIBYLS Web page .