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Scientific discipline: Structural biology
| GENERAL BEAMLINE INFORMATION |
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Operational
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Yes
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| Proposal cycle |
Proposals for Structural Biology Beamlines (2-month cycle) |
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Source characteristics
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Superbend
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Energy range
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5-17 keV (1% max flux)
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| Frequency range |
2.5-0.73 Angstrom wavelength |
| Beam size |
100 µm round beam default 10 µm and 30 µm collimators available for small samples with flux reduced to 1% and 12%, respectively. 120 µm x 120 µm Gaussian uncollimated beam shape at sample.
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| Scientific discipline |
Structural biology |
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Monochromator #1
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Si(111) Double crystal
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Calculated flux (1.9 GeV, 400 mA)
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2.5 x 1011 photons/sec at 11 keV
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Resolving power (E/ΔE)
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7000
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Monochromator #2
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0.6% bandpass multilayers
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Calculated flux (1.9 GeV, 400 mA)
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4 x 1013 photons/s with a convergence angle of 3 mrad
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Resolving power (E/ΔE)
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100
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Special notes
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Computers for data processing and analysis are available.
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Local contacts
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Physical Biosciences Division, Berkeley Lab Phone: (510) 486-5378 Fax: (510) 486-5298
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Physical Biosciences Division, Berkeley Lab Phone: (510) 495-2697 Fax: (510) 486-5298
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Physical Biosciences Division, Berkeley Lab Phone: (510) 486-5378 Fax: (510) 486-5298
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Spokesperson
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The Scripps Research Institute Phone: (858) 784-8119 Fax: (858) 784-2289
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| Beamline phone number |
(510) 495-2134 |
| Website |
SIBYLS: http://bl1231.als.lbl.gov/  |
| ENDSTATION INFORMATION |
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Endstation name
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Macromolecular crystallography
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Detector
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3 x 3 array CCD, ADSC Q315
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| Spatial resolution |
~1 µm pixel video microscope |
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Calculated spot size at sample (FWHM)
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10-100 µm (collimated)
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Sample format
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Single-crystal biomolecules
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Sample preparation
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Support labs available (Bldg. 6, Rm. 2105)
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Sample environment
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100-300K
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Scientific applications
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Macromolecular crystallography, multiple-wavelength anomalous diffraction (MAD)
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| ENDSTATION INFORMATION |
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Endstation name
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Small-angle X-ray scattering
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Detector
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Mar 165 CCD
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Calculated spot size at sample (FWHM)
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~5.0 x 0.5 mm
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Sample format
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~15 µL solutions of macromolecules and buffer blanks
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Sample preparation
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Support labs available (Bldg. 6, Rm. 2105)
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Sample environment
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80 - 4C, anaerobic
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Scientific applications
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High-throughput solution structural analyses by SAXS. Solution structure modeling, molecular envelope shape reconstruction, identification and characterization of oligomeric states and macromolecular complex assembly.
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More information about Beamline 12.3.1 is available on the SIBYLS Web page .
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